2 edition of Studies of lymphoid cell cytotoxicity in tissue culture. found in the catalog.
Studies of lymphoid cell cytotoxicity in tissue culture.
Richard Kempe Pitcairn
Written in English
|The Physical Object|
|Pagination||vii, 53 l.|
|Number of Pages||53|
Cloning of PD1 (REF. ) Perforin and granzymes shown to cooperate for cytotoxicity () T cells shown to be necessary and sufficient for cytotoxicity by sensitized lymphoid cells. A typical clonogenic survival experiment using adherent cells lines involves three distinct components, 1) treatment of the cell monolayer in tissue culture flasks, 2) preparation of single cell.
Lymphoid tissue has several different structural organizations related to its particular function in the immune response. The most highly organized lymphoid tissues are in the thymus and lymph nodes, which are well-defined encapsulated organs with easily identifiable architectures. In the spleen (a soft, purplish organ lying high in the abdomen), the lymphoid tissue is a cylinder of loosely. Organ Culture Lymphoid Tissue Lymphoid Cell Lymphoid Organ Reticular Cell These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Mesenchymal cells supported primary ALL cell survival in vitro: in cultures not exposed to exogenous T cells, recovery of leukemic cells from the five patients after 5 days of culture . In this study we have analyzed what types of cells transport tumor cell antigens to secondary lymphoid tissues. We show that neutrophils, monocytes and macrophages but not DCs engulf X-irradiated P leukemic cells after their injection into the peritoneal cavity, and that neutrophils and monocytes but not macrophages migrate to the parathymic.
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Initial studies on the properties of a bovine lymphoid cell culture line infected with theileria parva. either by a fluorescent technique or by antibody-dependent cell-mediated cytotoxicity.
There was no detectable surface immunoglobulin or secretion of immunoglobulins into the tissue culture by: Lysis of tissue culture cells (Chang cells) in the presence of PHA or antiserum to target cell antigens was quantitated by [51 Cr]chromate release.
Lymphocytes from lymph were at best poorly cytotoxic to antibody-treated target cells under conditions where purified blood lymphocytes from the same donors had normal lytic by: 8. The cytotoxic effect of guinea-pig lymphoid cells on guinea-pig hepatoma cell lines in tissue culture was investigated, using the microplate technique of Takasugi and Klein ().
The effect of lymphoid cells from guinea-pigs immunized against tumor cells was compared to that of cells Author: M Andjargholi, M M Dale. Masahisa Jinushi, Glenn Dranoff, in Cancer Immunotherapy, 1 Perforin. Cytotoxicity triggered by CD8 + T cells through the pfp-granzyme pathways is critical to tumor suppression (Smyth et al., b).The pfp-deficient mice display an enhanced susceptibility to chemical carcinogenesis and spontaneously develop lymphomas.
These tumors are rejected upon transplantation to wild-type. Ginsburg, H., and L. Sachs: Destruction of mouse and rat embryo cells in tissue culture by lymph node cells from unsensitized rats.
cell. comp. Physiol. 66, (). CrossRef Google Scholar Hirschhorn, K., R. Schreibman, S. Verbo, and R. Gruskin: The action of streptolysin S on peripheral lymphocytes of normal subjects and patients Cited by: 6.
Holm G. In vitro cytotoxic effects of lymphoid cells from rats with experimental autoimmune nephrosis. Clin Exp Immunol. Jan; 1 (1)– [PMC free article] Holm G, Perlmann P. Phytohaemagglutinin-induced cytotoxic action of unsensitized immunologically competent cells on allogeneic and xenogeneic tissue culture cells.
Nature. In another study , the CHCl 3 fraction of C. sempervirens demonstrated a significant cytotoxicity against HeLa cells. The cytotoxicity of the essential oil of the plant was tested on HepG2, Hep3B, A, MCF-7, and MDA-MB cancer cells, and it showed much less cytotoxicity against these cell.
The lymphoid cell line derived from the peripheral blood had a normal male karyotype. The lymphoid cell line derived from a biopsy specimen had a mode of 46 and a deleted chromosome 7 marker. Both lymphoid cell lines had B-cell characteristics. These autochthonous cell lines have been used for immunological studies in cytotoxicity assays and immu.
The ability of specifically immunized lymphoid cells to kill H-2 incompatible target tumor cells in tissue culture was shown to depend on the source of the lymphoid tissue (spleen versus lymph nodes). Marked cytotoxic effects were obtained with regional lymph node cells 7 to 10 days after primary immunization, whereas spleen cells from the same animals had little or no effect.
Cytotoxicity is one of the most important indicators for biological evaluation in vitro studies. In vitro, chemicals such as drugs and pesticides have different cytotoxicity mechanisms such as destruction of cell membranes, prevention of protein synthesis, irreversible binding to receptors etc.
In order to determine the cell death caused by these damages, there is a need for cheap, reliable. of the Tissue Culture Association in (Fedoroff, ).
This nomenclature was subsequently revised in (Schaeffer, ). Primary cell cultures, cell lines and cell strains have been defined as follows. A primary cell culture is 'a culture started from cells, tissues or organs taken directly from organisms. Tissue culture involves the in vitro maintenance and propagation of cells in optimal conditions.
Culturing animal cells, tissue or organs in a controlled artificial environment is called animal tissue culture. The importance of animal tissue culture was initially realized during the development of the polio vaccine using primary monkey kidney cells (the polio vaccine was the first commercial.
LEPOW IH, ROSS A. Studies on immune cellular injury. Functional role of C'1 esterase in immune cytotoxicity. J Exp Med. Dec 1; – [PMC free article] MITCHISON NA. Studies on the immunological response to foreign tumor transplants in the mouse. The role of lymph node cells in conferring immunity by adoptive transfer.
POTENTIATION OF CYTOTOXIC RESPONSES TO LYMPHOMA CELLS TABLE 3 Determination of Potentiating Activity in Different Lymphoid Tissues" CML" Percentage specific Source of cells PPD (10 ju.g/ml) cpm ± SE cytotoxicity BCG immune spleen Yes ± 75 84 No ± 53 BCG immune thymus Yes ± 48 91 No ± 60 52 BCG immune lymph node.
Holm, G. & Perlmann, P. Quantitative studies on phytohaemagglutinin-induced cytotoxicity by human lymphocytes against homologous cells in tissue culture.
Immunol. Meier, D. et al. Ectopic lymphoid-organ development occurs through interleukin 7-mediated enhanced survival of lymphoid-tissue-inducer cells.
Immun – (). CAS PubMed Google Scholar. A human CDexpressing target cell was required to study the influence of FCGR3A polymorphism on the rituximab-dependent CD16+ effector cell-mediated cytotoxicity in vitro.
The Burkitt lymphoma cell line Daudi was chosen for several reasons, although Daudi cells are not representative of NHL cells. BRYAN BALLANTYNE, in Toxicology of Organophosphate & Carbamate Compounds, 5 CYTOTOXICITY ASSAYS.
Cytotoxicity assays measure loss of some cellular or intercellular structure and/or functions, including lethal cytotoxicity. They thus give an indication of the potential to cause cell and tissue injury and as such have been used by some investigators to predict tissue injury.
Viruses could also escape destruction in locations where T cells are absent, such as proposed T-cell-free areas including the B-cell zone of immune follicles in lymph nodes.
Studies. used for cell counts after trypsinization, and some were used for a clonogenic assay. To measure uptake, cells were washed three times with tissue culture medium and then harvested with 50 ml M NaOH, which was collected with a cotton swab.
For the clonogenic assay, cells from a single well were trypsinized and suspended in ml medium. Specialized tissues that are components of the lymphatic system.
They provide fixed locations within the body where a variety of LYMPHOCYTES can | Explore the latest full-text research PDFs.
Second, in contrast to the short half-life of Fluc, marine luciferases used in this study are highly stable in cell culture media (t 1/2 > 24 hours). Therefore, the Matador assay is ideal for most.Summary. 5I Cr-release assay showed that peripheral blood lymphocytes from all of 21 normal individuals had cell-mediated cytotoxic reactivity against several human lymphoid tissue-culture cell lines.
Lysis by the normal donor lymphocytes of the various cell lines varied markedly among lines; lysis of a given cell line by an individual's lymphocytes also varied at different times.